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For high-fat diet experiments, mice were fed a 60 kcal% fat high-fat diet (Research Diets, D12451i) following either tamoxifen or AAV-TBG-Cre injection for 2-3 weeks

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Thus, survival and normal functionality of cells are critically dependent on oxygen delivery performed by the heme-containing hemoglobin protein, which is the most abundant protein in erythrocytes [43]